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A:
Those lines are for progress. It’s not fixed, but it keeps track of current progress. After you press enter, it will continue the installation.
Detection and quantification of cancer-related protein alterations with an MS-based assay and a prototype, DNA-directed heterobifunctional cross-linker.
Direct analysis of biological samples with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOFMS) typically requires sample preparation techniques such as tryptic digestion. However, this can alter the protein structure of some proteins, or even prevent the detection of certain proteins. This can be problematic, as unique and specific protein alterations are frequently observed in neoplastic cells. Consequently, identification of cancer-related protein alterations is not always possible. Here we present a method that can circumvent these limitations. This method uses biotinylated DNA that is specifically targeted to breast cancer-related genes, and that captures proteins from cell lysates. Using streptavidin-coated magnetic beads and a prototype DNA-directed heterobifunctional cross-linker, we selectively cross-link DNA-biotinylated proteins with their related DNA sequence, resulting in a single cross-linked DNA-protein complex. The biotinylated DNA was attached to magnetic beads by means of a biotinylated oligonucleotide designed to bind to complementary sequences in the biotinylated DNA. The cross-linker was covalently attached to the biotin molecule at the 5′-end, and to a diol or a primary amine group present on streptavidin-coated magnetic beads. This method has been demonstrated in breast cancer cell lines (MCF-7, ZR-75-1 and BT-474), and normal (HEL) and telomerase-negative (DU-145) cell lines. In some cases, cross-linking experiments can be directly performed on cell lysates. This method provides unique advantages over other methods, and can be used as a rapid and nondestructive method for identifying and quantifying proteins and protein alterations present in cellular samples.Q:
NHibernate MappingException : No persister for class for generated table
I am using NHibernate to write some test code to see how it works.
I have a class TestClass defined in a separate assembly, called Test.Data.
TestClass has
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The number appends across different devices….
A:
For some reason, the com.apple.security.temporary-exception.network.configuration.csrf.ssh-disable flag is enabled on my Macbook Air, but only on connect to a pair of specific wireless networks. The network is encrypted with WPA2-AES, the network is temporary in nature, and the network is not accessible from the Internet. I found this flag to be interesting as I am unable to connect my Air to the aforementioned network even through my iPhone 4 for example.
According to this document at Apple Support, in the keychain system there should be “multiple private keys” for the following accounts:
IDEs, account password, Network Keychain, Certificate/Key, and Find My
iPhone. These account keys may reside on the device, in the iCloud Key
Chain, in the Physical Keychain, or on a server such as the Apple
Developer Web Server (ADWS).
I have created a private key to specify, but I am not sure if the private key associated with this network specifically is found in a specific location. You can find my answer here. If you take a look at the iCloud keychain, you can see the temporary network keys:
If you use Terminal.app, you can view the list of the private keys, keychain items, etc. as follows:
If you are very much interested in understanding these keys further, you could simply read the Access Control entry inside of the core network.pem file associated with the temporary connection. I would also suggest you take a look at Apple’s Connection Sharing Guide.
It is worth nothing that these keys are temporary since Apple support states the following in the CSRFKS reference:
These network connections can be disabled for a limited time in the
configuration pane of Network.
From my research, this flag can be disabled through a third party application such as Airport Utility or network-config (as mentioned in your question).
You can also uninstall the keychain software.
Q:
How to properly position div relative to sibling (position:absolute)
I am working on a site with a fixed header that has a tiled background. In order to make the tiled background repeat, I have had to add a div, overlay-wrapper, outside of the header. I have the following markup:
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