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Endometriosis is a gynecological disease associated with a variety of symptoms including pelvic pain, deep dyspareunia, infertility and bowel and bladder dysfunction. It is characterized by the proliferation and invasion of endometrial tissue. Although the etiology of endometriosis remains to be determined, it is associated with an inflammatory microenvironment that appears to trigger the occurrence of ovarian endometriosis. A detailed understanding of the biology of endometrial cells is therefore crucial to the development of more effective therapeutic strategies. In this study, the motility and invasion of endometrial adenocarcinoma cells (AE-1 and AN3CA) were investigated. The Rho family of small GTPases are key signaling molecules involved in cell motility and invasion. We show here that the expression of RhoG, RhoE, Rac1 and Cdc42 are increased in endometriotic adenocarcinoma cells compared to benign endometrial cells. Both inhibition of RhoG and RhoE activity in endometrial adenocarcinoma cells and depletion of RhoE in stromal cells using a siRNA approach affect the motility and invasion of these cells in
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