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In recent years there has been an increase in the reported incidence of Clostridium difficile-associated diarrhoea. The isolation and detection of C. difficile is usually by culture techniques, but molecular methods have been developed in recent years. This study aimed to examine the usefulness of PCR-RFLP analysis of C. difficile clinical isolates from two hospitals in Northern Ireland. C. difficile was cultured from two areas of a general hospital and from a private clinic. Strains were screened for by phenotypic methods and by multiplex PCR. PCR products were digested with three restriction enzymes. Two additional samples contained unusual restriction patterns. Isolates were from several sites, including surgical wound, respiratory tract, wound, urine and faeces, and from both hospitals. This study confirmed the presence of three restriction profiles (ribotypes) and of two novel restriction patterns. Only two of the three ribotypes were found in both hospitals, whereas the other was isolated from only one hospital. PCR-RFLP analysis of C. difficile is a rapid, relatively inexpensive alternative to culture for the detection of C. difficile in healthcare settings. The study showed that different ribotypes of C. difficile were present in various clinical sites, suggesting multiple sources of infection.1999–2000 Formula Ford 2000 season
The 1999–2000 Formula Ford 2000 season was the 34th British Formula Ford season. It began on 6 September 1999 and ended on 8 May 2000. 18 different teams, 35 different drivers competed over 23 rounds.
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