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…The functional role of STAT6 in the development of anergy in human and murine T cells.
The suppressor of cytokine signaling 6 (SOCS6) is a member of a family of negative regulators of Janus kinase and signal transducers and activators of transcription (JAK/STAT) signaling pathways that inhibit cytokine and growth factor responses. The role of SOCS6 in the regulation of immune responses in the absence of cytokine stimulation is not fully understood. Here, we provide evidence for a functional role of SOCS6 in the development of anergy in human and murine CD4(+) T cells. By overexpression of SOCS6 in IL-2-dependent murine T cell hybridoma cells, we show that SOCS6 promotes both the development and maintenance of anergy in murine CD4(+) T cells. We demonstrate that SOCS6 is required for the development of T cell anergy in two murine models of T cell-mediated autoimmune diseases, experimental autoimmune encephalomyelitis and collagen-induced arthritis. Moreover, we show that SOCS6, in contrast to the STAT6alpha isoform, does not alter the phosphorylation status of phospholipase Cgamma-1 (PLCgamma-1). We also show that IL-4, one of the cytokines that can induce the expression of SOCS6, does not regulate the anergic phenotype of T cells. These results show that SOCS6 regulates the development of anergy in T cells and suggest that SOCS6 might regulate a network of pathways leading to T cell anergy.A new flavonoid glycoside from Alpinia oxyphylla.
A new compound, named alpinoside (1), together with three known flavonoids, quercetin-3-O-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside (2), quercetin-3-O-α-L-arabinopyranosyl-(1→6)-β-D-galactopyranoside (3) and myricetin-3-O-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside (4) were isolated from the stems of Alpinia oxyphylla, using a combination of various chromatographic methods. The structures of 1

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