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CD6 is a cell surface molecule and the human homologue of rat CD5. CD6 is one of the three co-stimulatory molecules that are expressed on T cells and interacting with other coreceptors CD7, CD28, and CD95. CD6 interacts with the cytoplasmic domain of the T cell receptor. CD6 also has a potential role in T cell signaling.

Effects

CD6, in conjunction with other coreceptors, can modulate the T cell response. CD6 ligation results in the calcium flux (in T-helper cells) and the activation of multiple signaling molecules, including the protein tyrosine kinases ZAP-70 and Syk, and the adapter molecule SLP-76. CD6-deficient mice have a reduced number of peripheral T cells and a failure of T-cell homeostasis in spleen and thymus. As such, CD6 plays a role in the development of T cells. CD6 is expressed on thymocytes and all mature T cells.

T cell activation

The mechanism by which CD6 enhances T cell activation is still unknown. CD6 is part of the coreceptor complex for T cell activation. The coreceptor CD28 enhances T cell proliferation and cytokine production via interactions with CD80/CD86, whereas CD6 binds to a different region of CD28 than CD80/CD86. CD6 and CD28 are expressed on activated T cells (e.g., after interaction with antigen-presenting cells), but CD6 can be also be expressed on resting T cells, as well as CD4 and CD8-deficient T cells.

In addition to its role in T cell activation, CD6 binds to the coreceptor CD72 on APC, which promotes the maturation and activation of B cells. CD72 is expressed on the surface of activated dendritic cells, and its expression is reduced following maturation or deactivation of dendritic cells. CD72 is also expressed by B cells at all stages of B cell development. CD72 is expressed by human B cells during terminal differentiation to plasma cells.

Coreceptor

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In a small bowl, whisk together the flour, sugar, baking powder, baking soda and salt.

In the bowl of a stand mixer, with a paddle attachment, beat the butter and sugar until pale and creamy, 3 to 5 minutes.

Add the eggs one at a time, beating well after each addition.

Slowly add the dry ingredients and milk to the butter mixture, alternating between the two and beating until incorporated.

Remove the bowl from the stand mixer and set it to one side.

With a spoon, gradually incorporate the melted chocolate into the batter, beating well after each addition.

In a large microwaveable bowl, melt the chopped chocolate in the microwave.

Spoon about a third of the batter into the bowl.

Quickly swirl the bowl to coat the bottom and sides of the bowl, then remove the bowl from the microwave.

Spoon the remaining batter into the bowl, along with the remaining chocolate.

Return the bowl to the microwave for 2 to 3 minutes at a time, stirring well at the end, until the bowl feels warm to the touch. Remove from the microwave and quickly stir to mix.

Pour into the cooled and lined pan.

Cover and refrigerate for about 2 hours, or until firm.

Invert the brownies onto a cutting board and remove the parchment paper.

Use a large sharp knife to cut the brownies into 12 pieces.

Dust with confectioner’s sugar to serve.

Serves 12

Originally published in AllRecipes.com. Updated by Kitchen Basics for Yahoo! Voices.Q:

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