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Interphase FISH
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FISH was performed as previously described \[[@B34]\]. FISH experiments were performed on metaphase spreads and interphase nuclei from wt and *p53*^*-/-*^cells. Probes were made from a PCR clone for the ***BRAC1***gene (RP11-4P17) in pBeloBACter-1 (Genome Systems, St Louis, MO) using BAC ends (Invitrogen, Carlsbad, CA). BAC DNA was isolated from the BAC-CEP 17 1.2Mb contig using the NucleoSpin DNA/RNA isolation kit (Macherey-Nagel, Bethlehem, PA). For *BRAC1*FISH, 5 μg of pBeloBACter-1 DNA (0.5 μg/μl) was labeled using a nick translation kit (Dyomics, Jena, Germany) according to the manufacturer’s instructions. A digoxigenin-labeled riboprobe was synthesized using the DIG nick translation kit (Sigma, St Louis, MO) according to the manufacturer’s instructions. FISH was performed on metaphase spreads
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