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Q:
Password field dialog, mac
I’m trying to implement a password field dialog in Mac OS X. If the user enters a password longer than 8 characters, it should only be shown if the user is activating the dialog.
Do I need to implement a KeychainCallback or something like that, or is there a better way?
A:
If you are using an Aqua UI in Mac OS X, then use the KCCKDFailureAction, it is the same mechanism used in the OK/Cancel dialogs in the System Preferences app. You just need to provide a callback method to be invoked when the keychain item fails to be created. The OS will handle that call in the System Preferences app for you. In it’s place you should just invoke a custom NSTableView row selection.
Delivery of small interfering RNA to endothelial cells using a retrovirus.
We report the development of a stable retroviral delivery system for the intracellular delivery of small interfering RNA (siRNA) in the endothelial cell line HUVEC. A pMX-based retroviral vector carrying a CMV-driven tetracycline-responsive promoter driving expression of either an unmodified or a 2′-O-methyl phosphorothioate (2′-OMe) modified siRNA against vascular endothelial growth factor (VEGF) was used to transduce HUVEC. Transduction with tetracycline-regulated retroviral vectors resulted in constitutive expression of the transgene mRNA for 4-5 days. SiRNA-mediated suppression of the target gene was accomplished by addition of doxycycline to the culture medium. Endothelial cell function was assessed using a Matrigel assay and cellular sprouting from a vessel lined with endothelium
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